CPR ELISA Kit (Human) (GWB-KBBFU6) (Aviva Cat. No. OKDD00204)

This exact product is available to purchase through Aviva Systems Biology.
Please see item number OKDD00204 to view product details and for ordering information.


Aviva Cat. No.: OKDD00204

Additional Information

Name CPR ELISA Kit (Human) (GWB-KBBFU6)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.06 ng/mL
Sample Type Serum, Plasma, Tissue homogenates, Cell lysates
Kit Detection Absorbance, OD 450 nm
Gene Symbol CPR
Gene id 5447
Alias Symbols CPR, CYPOR, DKFZp686G04235, FLJ26468, NADPH--cytochrome P450 reductase, P450R
Swissprot ID P16435
Protein Name NADPH--cytochrome P450 reductase
Description of Target This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes
Kit Principle Genway Biotech CPR ELISA Kit (Human) (GWB-KBBFU6) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CPR has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CPR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CPR captured in the well.
Kit Component
Anti-CPR Microplate96 Wells (12 x 8 Well Strips)
CPR Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X CPR Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_000932.3
Nucleotide Accession Num NM_000941.2
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBFU6
Species Reactivity Human