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CPT1B ELISA Kit (Human) (GWB-KBBFU8)

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GWB-KBBFU8

96 Wells
$659.00
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Additional Information

Name CPT1B ELISA Kit (Human) (GWB-KBBFU8)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.125 ng/mL
Sample Type tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CPT1B
Gene id 1375
Alias Symbols Carnitine O-palmitoyltransferase 1, muscle isoform, Carnitine O-palmitoyltransferase I, muscle isoform, Carnitine palmitoyltransferase 1B, Carnitine palmitoyltransferase I-like protein, CPT1M, CPT1-M, CPTI, CPT I, CPTI-M, FLJ55729, FLJ58750, KIAA1670, MCC
Swissprot ID Q92523
Protein Name carnitine O-palmitoyltransferase 1, muscle isoform
Description of Target The protein encoded by this gene, a member of the carnitine/choline acetyltransferase family, is the rate-controlling enzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the net transport of long-
Kit Principle Genway Biotech CPT1B ELISA Kit (Human) (GWB-KBBFU8) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CPT1B has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CPT1B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CPT1B captured in the well.
Kit Component
ComponentAmount
Anti-KIF5A Microplate96 Wells (12 x 8 Well Strips)
KIF5A Lyophilized Standard2 x 20 ng
100X Biotinylated KIF5A Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001138606
Nucleotide Accession Num NM_001145134
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBFU8
Species Reactivity Human