Cr2 ELISA Kit (Rat) (GWB-KBBXK1) (Aviva Cat. No. OKCD00665)

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Please see item number OKCD00665 to view product details and for ordering information.


GWB-KBBXK1

Aviva Cat. No.: OKCD00665

Additional Information

Name Cr2 ELISA Kit (Rat) (GWB-KBBXK1)
Kit Range 1.56 - 100 ng/mL
Sensitivity < 0.57 ng/mL
Sample Type serum, plasma and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CR2
Gene id 289395
Alias Symbols CD21, C3DR, Complement Component(3d/Epstein Barr Virus)Receptor 2, Complement C3d receptor, Epstein-Barr virus receptor, EBV receptor
Swissprot ID D3Z9F7
Protein Name complement receptor type 2
Kit Principle Genway Biotech Cr2 ELISA Kit (Rat) (GWB-KBBXK1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cr2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cr2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cr2 captured in the well.
Kit Component
ComponentAmount
Anti-PRL Microplate96 Wells (12 x 8 Well Strips)
PRL Lyophilized Standard2 x 100 ng
100X Biotinylated PRL Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001099459
Nucleotide Accession Num NM_001105989
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)98-105%97-105%78-97%95-103%
EDTA Plasma (n=5)97-105%79-95%94-102%93-101%
Heparin Plasma (n=5)96-103%78-97%78-104%88-101%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)87-10491
EDTA Plasma (n=5)87-10497
Heparin Plasma (n=5)98-105102
Datasheets/Manuals Printable datasheet for GWB-KBBXK1
Species Reactivity Rat