| Name |
CREBBP ELISA Kit (Human) (GWB-KBBCQ5) |
| Kit Range |
0.312 - 20 ng/mL |
| Sensitivity |
< 0.132 ng/mL |
| Sample Type |
tissue homogenates and other biological fluids. |
| Kit Detection |
Colorimetric, OD 450 nm |
| Gene Symbol |
CREBBP |
| Gene Fullname |
CREB binding protein |
| Gene id |
1387 |
| Alias Symbols |
CBP, CREB-binding protein, KAT3A, RSTS, RTS |
| Swissprot ID |
Q92793 |
| Protein Name |
CREB-binding protein |
| Kit Principle |
Genway Biotech CREBBP ELISA Kit (Human) (GWB-KBBCQ5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CREBBP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CREBBP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CREBBP captured in the well. |
| Kit Component |
| Component | Amount |
|---|
| Anti-PCSK2 Microplate | 96 Wells (12 x 8 Well Strips) | | PCSK2 Lyophilized Standard | 2 x 20 ng | | 100X Biotinylated PCSK2 Detector Antibody | 120 uL | | Avidin/HRP Conjugate | 120 uL | | Standard Diluent | 1 x 20 mL | | Detector Antibody Diluent | 1 x 12 mL | | Conjugate Diluent | 1 x 12 mL | | 30X Wash Buffer | 1 x 20 mL | | TMB Substrate | 1 x 9 mL | | Stop Solution | 1 x 6 mL |
|
| Protein Accession Num |
NP_001073315 |
| Nucleotide Accession Num |
NM_001079846 |
| Assay Info |
Assay Methodology: Quantitative Sandwich Immunoassay |
| Specificity |
This assay has high sensitivity and excellent specificity for detection of CREB Binding Protein (CREBBP).
No significant cross-reactivity or interference between CREB Binding Protein (CREBBP) and analogues was observed. |
| Application |
ELISA |
| Reconstitution and Storage |
Store as indicated in product manual. |
| Kit Duration |
~ 3 Hours |
| Datasheets/Manuals |
Printable datasheet for GWB-KBBCQ5 |
| Species Reactivity |
Human |
