CREBBP ELISA Kit (Human) (GWB-KBBCQ5) (Aviva Cat. No. OKCD00928)

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Please see item number OKCD00928 to view product details and for ordering information.


GWB-KBBCQ5

Aviva Cat. No.: OKCD00928

Additional Information

Name CREBBP ELISA Kit (Human) (GWB-KBBCQ5)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.132 ng/mL
Sample Type tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CREBBP
Gene Fullname CREB binding protein
Gene id 1387
Alias Symbols CBP, CREB-binding protein, KAT3A, RSTS, RTS
Swissprot ID Q92793
Protein Name CREB-binding protein
Kit Principle Genway Biotech CREBBP ELISA Kit (Human) (GWB-KBBCQ5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CREBBP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CREBBP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CREBBP captured in the well.
Kit Component
ComponentAmount
Anti-PCSK2 Microplate96 Wells (12 x 8 Well Strips)
PCSK2 Lyophilized Standard2 x 20 ng
100X Biotinylated PCSK2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001073315
Nucleotide Accession Num NM_001079846
Assay Info Assay Methodology: Quantitative Sandwich Immunoassay
Specificity This assay has high sensitivity and excellent specificity for detection of CREB Binding Protein (CREBBP). No significant cross-reactivity or interference between CREB Binding Protein (CREBBP) and analogues was observed.
Application ELISA
Reconstitution and Storage Store as indicated in product manual.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBCQ5
Species Reactivity Human