CRY1 ELISA Kit (Human) (GWB-KBBTB1) (Aviva Cat. No. OKCD01672)

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Please see item number OKCD01672 to view product details and for ordering information.


Aviva Cat. No.: OKCD01672

Additional Information

Name CRY1 ELISA Kit (Human) (GWB-KBBTB1)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.129 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CRY1
Gene id 1407
Alias Symbols Cryptochrome-1, PHLL1
Swissprot ID Q16526
Protein Name Cryptochrome-1
Description of Target Transcriptional repressor which forms a core component of the circadian clock.
Kit Principle Genway Biotech CRY1 ELISA Kit (Human) (GWB-KBBTB1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CRY1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CRY1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CRY1 captured in the well.
Kit Component
Anti-CRY1 Microplate96 Wells (12 x 8 Well Strips)
CRY1 Lyophilized Standard2 x 20 ng
100X Biotinylated CRY1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_004066
Nucleotide Accession Num NM_004075.
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBTB1
Species Reactivity Human