Crybb2 ELISA Kit (Mouse) (GWB-KBBNJ7) (Aviva Cat. No. OKCD00968)

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Please see item number OKCD00968 to view product details and for ordering information.


GWB-KBBNJ7

Aviva Cat. No.: OKCD00968

Additional Information

Name Crybb2 ELISA Kit (Mouse) (GWB-KBBNJ7)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.125 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CRYBB2
Gene id 12961
Alias Symbols Aey2, betaB2-crystallin, Beta-B2 crystallin, Beta-crystallin B2, Beta-crystallin Bp, Cryb-2, Phil
Swissprot ID P62696
Protein Name beta-crystallin B2
Description of Target Crystallins are the dominant structural components of the vertebrate eye lens.
Kit Principle Genway Biotech Crybb2 ELISA Kit (Mouse) (GWB-KBBNJ7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Crybb2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Crybb2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Crybb2 captured in the well.
Kit Component
ComponentAmount
Anti-CPT1B Microplate96 Wells (12 x 8 Well Strips)
CPT1B Lyophilized Standard2 x 20 ng
100X Biotinylated CPT1B Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_031799
Nucleotide Accession Num NM_007773
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
79-90%93-101%87-94%
Kit Recovery
MatrixRecovery Range (%)Average (%)
88-9996
Datasheets/Manuals Printable datasheet for GWB-KBBNJ7
Species Reactivity Mouse