Crygf ELISA Kit (Mouse) (GWB-KBBOE9)

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96 Wells
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Additional Information

Name Crygf ELISA Kit (Mouse) (GWB-KBBOE9)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.055 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CRYGF
Gene id 12969
Alias Symbols 3110001K11Rik, Cryg-2, DGcry-2, Gamma-crystallin F, Gamma-F-crystallin, Len-2
Swissprot ID Q9CXV3
Protein Name gamma-crystallin F
Description of Target Crystallins are the dominant structural components of the vertebrate eye lens.
Kit Principle Genway Biotech Crygf ELISA Kit (Mouse) (GWB-KBBOE9) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Crygf has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Crygf is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Crygf captured in the well.
Kit Component
Anti-Fth1 Microplate96 Wells (12 x 8 Well Strips)
Fth1 Lyophilized Standard2 x 10 ng
100X Biotinylated Fth1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_081286
Nucleotide Accession Num NM_027010
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Kit Recovery
MatrixRecovery Range (%)Average (%)
Datasheets/Manuals Printable datasheet for GWB-KBBOE9
Species Reactivity Mouse