CSF2RB ELISA Kit (Human) (GWB-KBBMN5) (Aviva Cat. No. OKCD00838)

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Please see item number OKCD00838 to view product details and for ordering information.


GWB-KBBMN5

Aviva Cat. No.: OKCD00838

Additional Information

Name CSF2RB ELISA Kit (Human) (GWB-KBBMN5)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.113 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CSF2RB
Gene id 1439
Alias Symbols CD131, CDw131, Cytokine receptor common subunit beta, GM-CSF/IL-3/IL-5 receptor common beta subunit, IL3RB, IL5RB
Swissprot ID P32927
Protein Name Cytokine receptor common subunit beta
Description of Target High affinity receptor for interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor.
Kit Principle Genway Biotech CSF2RB ELISA Kit (Human) (GWB-KBBMN5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CSF2RB has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CSF2RB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CSF2RB captured in the well.
Kit Component
ComponentAmount
Anti-Itgam Microplate96 Wells (12 x 8 Well Strips)
Itgam Lyophilized Standard2 x 20 ng
100X Biotinylated Itgam Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_000386
Nucleotide Accession Num NM_000395
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBMN5
Species Reactivity Human