CSNK1d ELISA Kit (Human) (GWB-KBBJH3)

GWB-KBBJH3

96 Wells
$605.00
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Additional Information

Name CSNK1d ELISA Kit (Human) (GWB-KBBJH3)
Availability In Stock
Kit Range 0.156-10 ng/mL
Sensitivity < 0.052 ng/mL
Sample Type Serum, Plasma, Tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol CSNK1d
Gene id 1453
Alias Symbols Casein kinase I isoform delta, CKId, CKI-delta, HCKID
Swissprot ID P48730
Protein Name Casein kinase I isoform delta
Kit Principle Genway Biotech CSNK1d ELISA Kit (Human) (GWB-KBBJH3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CSNK1d has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CSNK1d is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CSNK1d captured in the well.
Kit Component
ComponentAmount
Anti-CSNK1d Microplate96 Wells (12 x 8 Well Strips)
CSNK1d Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X CSNK1d Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001884.2
Nucleotide Accession Num NM_001893.4
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBJH3
Species Reactivity Human