CTRB2 ELISA Kit (Human) (GWB-KBBHA6)

GWB-KBBHA6

96 Wells
$580.00
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Additional Information

Name CTRB2 ELISA Kit (Human) (GWB-KBBHA6)
Kit Range 1.56 - 100 ng/mL
Sensitivity < 0.62 ng/mL
Sample Type serum, plasma and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CTRB2
Gene id 440387
Swissprot ID Q6GPI1
Protein Name chymotrypsinogen B2
Kit Principle Genway Biotech CTRB2 ELISA Kit (Human) (GWB-KBBHA6) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CTRB2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CTRB2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CTRB2 captured in the well.
Kit Component
ComponentAmount
Anti-SLC7A9 Microplate96 Wells (12 x 8 Well Strips)
SLC7A9 Lyophilized Standard2 x 100 ng
100X Biotinylated SLC7A9 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001020371
Nucleotide Accession Num NM_001025200
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)89-103%88-104%80-99%83-96%
EDTA Plasma (n=5)97-105%80-101%85-96%80-93%
Heparin Plasma (n=5)79-103%90-103%99-105%88-98%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)87-10395
EDTA Plasma (n=5)90-10497
Heparin Plasma (n=5)88-9992
Datasheets/Manuals Printable datasheet for GWB-KBBHA6