Cxcl8 ELISA Kit (Horse) (GWB-KBBRL2) (Aviva Cat. No. OKCD00740)

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Please see item number OKCD00740 to view product details and for ordering information.


GWB-KBBRL2

Aviva Cat. No.: OKCD00740

Additional Information

Name Cxcl8 ELISA Kit (Horse) (GWB-KBBRL2)
Kit Range 31.25 - 2,000 pg/mL
Sensitivity < 13.5 pg/mL
Sample Type serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CXCL8
Gene id 100037400
Alias Symbols CXCL8, AMCF-I, GCP1, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP1, SCYB8, TSG1, B-ENAP, Neutrophil-Activating Protein 1, Granulocyte Chemotactic Protein 1
Swissprot ID O62812
Protein Name interleukin-8
Description of Target IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus (
Kit Principle Genway Biotech Cxcl8 ELISA Kit (Horse) (GWB-KBBRL2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cxcl8 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cxcl8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cxcl8 captured in the well.
Kit Component
ComponentAmount
Anti-ELANE Microplate96 Wells (12 x 8 Well Strips)
ELANE Lyophilized Standard2 x 2 ng
100X Biotinylated ELANE Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001077420
Nucleotide Accession Num NM_001083951
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)78-96%79-101%88-98%98-105%
EDTA Plasma (n=5)83-95%79-98%94-102%85-101%
Heparin Plasma (n=5)82-92%86-102%97-105%79-102%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)95-105102
EDTA Plasma (n=5)82-10195
Heparin Plasma (n=5)85-9288
Datasheets/Manuals Printable datasheet for GWB-KBBRL2
Species Reactivity Horse