Cxcl8 ELISA Kit (Monkey) (GWB-KBBIW5) (Aviva Cat. No. OKCD00142)

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Please see item number OKCD00142 to view product details and for ordering information.


GWB-KBBIW5

Aviva Cat. No.: OKCD00142

Additional Information

Name Cxcl8 ELISA Kit (Monkey) (GWB-KBBIW5)
Kit Range 15.62 - 1,000 pg/mL
Sensitivity < 5.5 pg/mL
Sample Type serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CXCL8
Gene id 613028
Alias Symbols CXCL8, AMCF-I, GCP1, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP1, SCYB8, TSG1, B-ENAP, Neutrophil-Activating Protein 1, Granulocyte Chemotactic Protein 1
Swissprot ID P67813
Protein Name interleukin-8
Description of Target IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus (
Kit Principle Genway Biotech Cxcl8 ELISA Kit (Monkey) (GWB-KBBIW5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cxcl8 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cxcl8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cxcl8 captured in the well.
Kit Component
ComponentAmount
Anti-CXCL8 Microplate96 Wells (12 x 8 Well Strips)
CXCL8 Lyophilized Standard2 x 1 ng
100X Biotinylated CXCL8 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001028137
Nucleotide Accession Num NM_001032965
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)88-97%96-103%85-96%95-102%
EDTA Plasma (n=5)93-101%82-94%91-98%79-95%
Heparin Plasma (n=5)81-94%95-102%94-102%85-94%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)80-8882
EDTA Plasma (n=5)93-10196
Heparin Plasma (n=5)93-10299
Datasheets/Manuals Printable datasheet for GWB-KBBIW5
Species Reactivity Monkey