CYP24A1 ELISA Kit (Human) (GWB-KBBXB7) (Aviva Cat. No. OKCD01774)

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Aviva Cat. No.: OKCD01774

Additional Information

Name CYP24A1 ELISA Kit (Human) (GWB-KBBXB7)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.055 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CYP24A1
Gene id 1591
Alias Symbols 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial, 24-OHase, CP24, CYP24, Cytochrome P450 24A1, Cytochrome P450-CC24, MGC126273, MGC126274, P450-CC24, Vitamin D(3) 24-hydroxylase
Swissprot ID Q07973
Protein Name 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
Description of Target Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25-hydroxyvitamin D3) and calcitriol (1-alpha,25-dihydroxyvitamin D3). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading
Kit Principle Genway Biotech CYP24A1 ELISA Kit (Human) (GWB-KBBXB7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CYP24A1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CYP24A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CYP24A1 captured in the well.
Kit Component
Anti-CYP24A1 Microplate96 Wells (12 x 8 Well Strips)
CYP24A1 Lyophilized Standard2 x 10 ng
100X Biotinylated CYP24A1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_000773
Nucleotide Accession Num NM_000782
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBXB7
Species Reactivity Human