| Name |
CYP24A1 ELISA Kit (Human) (GWB-KBBXB7) |
| Kit Range |
0.156 - 10 ng/mL |
| Sensitivity |
< 0.055 ng/mL |
| Sample Type |
tissue homogenates, cell lysates and other biological fluids |
| Kit Detection |
Colorimetric, OD 450 nm |
| Gene Symbol |
CYP24A1 |
| Gene id |
1591 |
| Alias Symbols |
1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial, 24-OHase, CP24, CYP24, Cytochrome P450 24A1, Cytochrome P450-CC24, MGC126273, MGC126274, P450-CC24, Vitamin D(3) 24-hydroxylase |
| Swissprot ID |
Q07973 |
| Protein Name |
1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial |
| Description of Target |
Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25-hydroxyvitamin D3) and calcitriol (1-alpha,25-dihydroxyvitamin D3). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading |
| Kit Principle |
Genway Biotech CYP24A1 ELISA Kit (Human) (GWB-KBBXB7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CYP24A1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CYP24A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CYP24A1 captured in the well. |
| Kit Component |
| Component | Amount |
|---|
| Anti-CYP24A1 Microplate | 96 Wells (12 x 8 Well Strips) |
| CYP24A1 Lyophilized Standard | 2 x 10 ng |
| 100X Biotinylated CYP24A1 Detector Antibody | 120 uL |
| Avidin/HRP Conjugate | 120 uL |
| Standard Diluent | 1 x 20 mL |
| Detector Antibody Diluent | 1 x 12 mL |
| Conjugate Diluent | 1 x 12 mL |
| 30X Wash Buffer | 1 x 20 mL |
| TMB Substrate | 1 x 9 mL |
| Stop Solution | 1 x 6 mL |
|
| Protein Accession Num |
NP_000773 |
| Nucleotide Accession Num |
NM_000782 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Kit Duration |
~ 3 Hours |
| Datasheets/Manuals |
Printable datasheet for GWB-KBBXB7 |
| Species Reactivity |
Human |
