CYP26A1 ELISA Kit (Human) (GWB-KBBDF5) (Aviva Cat. No. OKCD01773)

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GWB-KBBDF5

Aviva Cat. No.: OKCD01773

Additional Information

Name CYP26A1 ELISA Kit (Human) (GWB-KBBDF5)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.055 ng/mL
Sample Type tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CYP26A1
Gene id 1592
Alias Symbols CP26, CYP26, Cytochrome P450 26A1, Cytochrome P450RAI, Cytochrome P450 retinoic acid-inactivating 1, hP450RAI, P450RAI, P450RAI1, Retinoic acid 4-hydroxylase, Retinoic acid-metabolizing cytochrome
Swissprot ID O43174
Protein Name Cytochrome P450 26A1
Description of Target Plays a key role in retinoic acid metabolism. Acts on retinoids, including all-trans-retinoic acid (RA) and its stereoisomer 9-cis-RA. Capable of both 4-hydroxylation and 18-hydroxylation. Responsible for generation of several hydroxylated forms of RA, in
Kit Principle Genway Biotech CYP26A1 ELISA Kit (Human) (GWB-KBBDF5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CYP26A1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CYP26A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CYP26A1 captured in the well.
Kit Component
ComponentAmount
Anti-CYP26A1 Microplate96 Wells (12 x 8 Well Strips)
CYP26A1 Lyophilized Standard2 x 10 ng
100X Biotinylated CYP26A1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_000774
Nucleotide Accession Num NM_000783
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBDF5
Species Reactivity Human