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CYP3A7 ELISA Kit (Human) (GWB-KBBMU1)

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GWB-KBBMU1

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Additional Information

Name CYP3A7 ELISA Kit (Human) (GWB-KBBMU1)
Description of Target Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xe
Kit Range 1.56 - 100 ng/mL
Sensitivity < 0.57 ng/mL
Sample Type tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CYP3A7
Gene id 100861540
Alias Symbols CP37, CYPIIIA7, Cytochrome P450 3A7, Cytochrome P450-HFLA, P450-HFLA
Swissprot ID P24462
Protein Name Cytochrome P450 3A7
Kit Principle Genway Biotech CYP3A7 ELISA Kit (Human) (GWB-KBBMU1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CYP3A7 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CYP3A7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CYP3A7 captured in the well.
Kit Component
ComponentAmount
Anti-ENDOD1 Microplate96 Wells (12 x 8 Well Strips)
ENDOD1 Lyophilized Standard2 x 100 ng
100X Biotinylated ENDOD1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_000756
Nucleotide Accession Num NM_000765
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
98-105%96-104%98-105%91-101%
Kit Recovery
MatrixRecovery Range (%)Average (%)
83-9286
Datasheets/Manuals Printable datasheet for GWB-KBBMU1
Species Reactivity Human