CYP4F2 ELISA Kit (Human) (GWB-KBBIZ4)

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GWB-KBBIZ4

96 Wells
$729.00
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Additional Information

Name CYP4F2 ELISA Kit (Human) (GWB-KBBIZ4)
Kit Range 1.56 - 100 ng/mL
Sensitivity < 0.59 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CYP4F2
Gene id 8529
Alias Symbols CYPIVF2, Cytochrome P450 4F2, Cytochrome P450-LTB-omega, Leukotriene-B(4) 20-monooxygenase 1, Leukotriene-B(4) omega-hydroxylase 1
Swissprot ID P78329
Protein Name Phylloquinone omega-hydroxylase CYP4F2
Description of Target Omega-hydroxylase that oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids and xenobiotics. Plays a key role in vitamin K catabolism by mediating omega-hydroxylation of vitamin K1 (phylloquinone), and menaquinone-4 (MK-
Kit Principle Genway Biotech CYP4F2 ELISA Kit (Human) (GWB-KBBIZ4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CYP4F2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CYP4F2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CYP4F2 captured in the well.
Kit Component
ComponentAmount
Anti-CYP4F2 Microplate96 Wells (12 x 8 Well Strips)
CYP4F2 Lyophilized Standard2 x 100 ng
100X Biotinylated CYP4F2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001073
Nucleotide Accession Num NM_001082
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBIZ4
Species Reactivity Human