CYSLTR1 ELISA Kit (Human) (GWB-KBBJS2) (Aviva Cat. No. OKCD01674)

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GWB-KBBJS2

Aviva Cat. No.: OKCD01674

Additional Information

Name CYSLTR1 ELISA Kit (Human) (GWB-KBBJS2)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.135 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CYSLTR1
Gene id 10800
Alias Symbols CysLT(1), CysLT1, CYSLT1, CYSLT1R, CYSLTR, CysLTR1, Cysteinyl leukotriene D4 receptor, Cysteinyl leukotriene receptor 1, G-protein coupled receptor HG55, HG55, HMTMF81, LTD4 receptor, MGC46139
Swissprot ID Q9Y271
Protein Name Cysteinyl leukotriene receptor 1
Description of Target Receptor for cysteinyl leukotrienes mediating bronchoconstriction of individuals with and without asthma. Stimulation by LTD4 results in the contraction and proliferation of smooth muscle, edema, eosinophil migration and damage to the mucus layer in the l
Kit Principle Genway Biotech CYSLTR1 ELISA Kit (Human) (GWB-KBBJS2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CYSLTR1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CYSLTR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CYSLTR1 captured in the well.
Kit Component
ComponentAmount
Anti-CYSLTR1 Microplate96 Wells (12 x 8 Well Strips)
CYSLTR1 Lyophilized Standard2 x 20 ng
100X Biotinylated CYSLTR1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001269115
Nucleotide Accession Num NM_001282186
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBJS2
Species Reactivity Human