| Name |
CYSLTR1 ELISA Kit (Human) (GWB-KBBJS2) |
| Kit Range |
0.312 - 20 ng/mL |
| Sensitivity |
< 0.135 ng/mL |
| Sample Type |
tissue homogenates, cell lysates and other biological fluids |
| Kit Detection |
Colorimetric, OD 450 nm |
| Gene Symbol |
CYSLTR1 |
| Gene id |
10800 |
| Alias Symbols |
CysLT(1), CysLT1, CYSLT1, CYSLT1R, CYSLTR, CysLTR1, Cysteinyl leukotriene D4 receptor, Cysteinyl leukotriene receptor 1, G-protein coupled receptor HG55, HG55, HMTMF81, LTD4 receptor, MGC46139 |
| Swissprot ID |
Q9Y271 |
| Protein Name |
Cysteinyl leukotriene receptor 1 |
| Description of Target |
Receptor for cysteinyl leukotrienes mediating bronchoconstriction of individuals with and without asthma. Stimulation by LTD4 results in the contraction and proliferation of smooth muscle, edema, eosinophil migration and damage to the mucus layer in the l |
| Kit Principle |
Genway Biotech CYSLTR1 ELISA Kit (Human) (GWB-KBBJS2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CYSLTR1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CYSLTR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CYSLTR1 captured in the well. |
| Kit Component |
| Component | Amount |
|---|
| Anti-CYSLTR1 Microplate | 96 Wells (12 x 8 Well Strips) |
| CYSLTR1 Lyophilized Standard | 2 x 20 ng |
| 100X Biotinylated CYSLTR1 Detector Antibody | 120 uL |
| Avidin/HRP Conjugate | 120 uL |
| Standard Diluent | 1 x 20 mL |
| Detector Antibody Diluent | 1 x 12 mL |
| Conjugate Diluent | 1 x 12 mL |
| 30X Wash Buffer | 1 x 20 mL |
| TMB Substrate | 1 x 9 mL |
| Stop Solution | 1 x 6 mL |
|
| Protein Accession Num |
NP_001269115 |
| Nucleotide Accession Num |
NM_001282186 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Kit Duration |
~ 3 Hours |
| Datasheets/Manuals |
Printable datasheet for GWB-KBBJS2 |
| Species Reactivity |
Human |
