DDO ELISA Kit (Human) (GWB-KBBBU8) (Aviva Cat. No. OKCD00559)

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Please see item number OKCD00559 to view product details and for ordering information.


GWB-KBBBU8

Aviva Cat. No.: OKCD00559

Additional Information

Name DDO ELISA Kit (Human) (GWB-KBBBU8)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.059 ng/mL
Sample Type tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol DDO
Gene id 8528
Alias Symbols DASOX, DDO-1, DDO-2
Swissprot ID Q99489
Protein Name D-aspartate oxidase
Description of Target The protein encoded by this gene is a peroxisomal flavoprotein that catalyzes the oxidative deamination of D-aspartate and N-methyl D-aspartate. Flavin adenine dinucleotide or 6-hydroxyflavin adenine dinucleotide can serve as the cofactor in this reaction
Kit Principle Genway Biotech DDO ELISA Kit (Human) (GWB-KBBBU8) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DDO has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DDO is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DDO captured in the well.
Kit Component
ComponentAmount
Anti-DAPP1 Microplate96 Wells (12 x 8 Well Strips)
DAPP1 Lyophilized Standard2 x 10 ng
100X Biotinylated DAPP1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_003640
Nucleotide Accession Num NM_003649
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBBU8
Species Reactivity Human