| Name |
DDO ELISA Kit (Human) (GWB-KBBBU8) |
| Kit Range |
0.156 - 10 ng/mL |
| Sensitivity |
< 0.059 ng/mL |
| Sample Type |
tissue homogenates and other biological fluids. |
| Kit Detection |
Colorimetric, OD 450 nm |
| Gene Symbol |
DDO |
| Gene id |
8528 |
| Alias Symbols |
DASOX, DDO-1, DDO-2 |
| Swissprot ID |
Q99489 |
| Protein Name |
D-aspartate oxidase |
| Description of Target |
The protein encoded by this gene is a peroxisomal flavoprotein that catalyzes the oxidative deamination of D-aspartate and N-methyl D-aspartate. Flavin adenine dinucleotide or 6-hydroxyflavin adenine dinucleotide can serve as the cofactor in this reaction |
| Kit Principle |
Genway Biotech DDO ELISA Kit (Human) (GWB-KBBBU8) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DDO has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DDO is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DDO captured in the well. |
| Kit Component |
| Component | Amount |
|---|
| Anti-DAPP1 Microplate | 96 Wells (12 x 8 Well Strips) | | DAPP1 Lyophilized Standard | 2 x 10 ng | | 100X Biotinylated DAPP1 Detector Antibody | 120 uL | | Avidin/HRP Conjugate | 120 uL | | Standard Diluent | 1 x 20 mL | | Detector Antibody Diluent | 1 x 12 mL | | Conjugate Diluent | 1 x 12 mL | | 30X Wash Buffer | 1 x 20 mL | | TMB Substrate | 1 x 9 mL | | Stop Solution | 1 x 6 mL |
|
| Protein Accession Num |
NP_003640 |
| Nucleotide Accession Num |
NM_003649 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Kit Duration |
~ 3 Hours |
| Datasheets/Manuals |
Printable datasheet for GWB-KBBBU8 |
| Species Reactivity |
Human |
