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96 Wells

Additional Information

Name DDX5 ELISA Kit (Human) (GWB-KBBHL5)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.062 ng/mL
Sample Type Serum, Plasma, Tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol DDX5
Gene id 1655
Alias Symbols DEAD box protein 5, DKFZp434E109, DKFZp686J01190, G17P1, HELR, HLR1, HUMP68, p68, RNA helicase p68
Swissprot ID P17844
Protein Name probable ATP-dependent RNA helicase DDX5
Description of Target DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and
Kit Principle Genway Biotech DDX5 ELISA Kit (Human) (GWB-KBBHL5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DDX5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DDX5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DDX5 captured in the well.
Kit Component
Anti-DDX5 Microplate96 Wells (12 x 8 Well Strips)
DDX5 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X DDX5 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001307524.1
Nucleotide Accession Num NM_001320595.1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBHL5
Species Reactivity Human