DEFA3 ELISA Kit (Human) (GWB-KBBJV4) (Aviva Cat. No. OKCD01808)

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GWB-KBBJV4

Aviva Cat. No.: OKCD01808

Additional Information

Name DEFA3 ELISA Kit (Human) (GWB-KBBJV4)
Kit Range 0.47 - 30 ng/mL
Sensitivity < 0.21 ng/mL
Sample Type serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol DEFA3
Gene id 1668
Alias Symbols DEF3, Defensin, alpha 3, HNP3, HNP-3, HP3, HP-3, Neutrophil defensin 3
Swissprot ID P59666
Protein Name Neutrophil defensin 3
Description of Target Defensin 2 and defensin 3 have antibiotic, fungicide and antiviral activities. Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane.
Kit Principle Genway Biotech DEFA3 ELISA Kit (Human) (GWB-KBBJV4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DEFA3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DEFA3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DEFA3 captured in the well.
Kit Component
ComponentAmount
Anti-DEFA3 Microplate96 Wells (12 x 8 Well Strips)
DEFA3 Lyophilized Standard2 x 30 ng
100X Biotinylated DEFA3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_005208
Nucleotide Accession Num NM_005217
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)89-103%80-92%86-95%82-97%
EDTA Plasma (n=5)82-93%98-105%97-105%81-104%
Heparin Plasma (n=5)78-97%84-104%90-98%87-95%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)95-105101
EDTA Plasma (n=5)78-9086
Heparin Plasma (n=5)88-9791
Datasheets/Manuals Printable datasheet for GWB-KBBJV4
Species Reactivity Human