Defa6 ELISA Kit (Mouse) (GWB-KBBHN7) (Aviva Cat. No. OKCD01807)

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Aviva Cat. No.: OKCD01807

Additional Information

Name Defa6 ELISA Kit (Mouse) (GWB-KBBHN7)
Kit Range 15.6 - 1,000 pg/mL
Sensitivity < 6.9 pg/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol DEFA6
Gene id 13240
Alias Symbols Defa12, Defcr12, Defcr6
Swissprot ID P50704
Protein Name Alpha-defensin 6/12
Description of Target Has broad-spectrum antimicrobial properties. Has antibacterial activity against the Gram-positive bacterium L.monocytogenes EGD and the Gram-negative bacteria E.coli ML-35p and avirulent S.typhimurium 7953, but not against the mouse-virulent S.typhimurium
Kit Principle Genway Biotech Defa6 ELISA Kit (Mouse) (GWB-KBBHN7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Defa6 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Defa6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Defa6 captured in the well.
Kit Component
Anti-DEFA6 Microplate96 Wells (12 x 8 Well Strips)
DEFA6 Lyophilized Standard2 x 1 ng
100X Biotinylated DEFA6 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_031878
Nucleotide Accession Num NM_007852
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)84-103%81-90%84-94%89-103%
EDTA Plasma (n=5)87-97%80-92%97-105%78-89%
Heparin Plasma (n=5)87-94%85-95%89-96%87-101%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)97-105101
EDTA Plasma (n=5)86-9893
Heparin Plasma (n=5)80-9979
Datasheets/Manuals Printable datasheet for GWB-KBBHN7
Species Reactivity Mouse