DIAPH1 ELISA Kit (Human) (GWB-KBBSV3) (Aviva Cat. No. OKDD00229)

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GWB-KBBSV3

Aviva Cat. No.: OKDD00229

Additional Information

Name DIAPH1 ELISA Kit (Human) (GWB-KBBSV3)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.051 ng/mL
Sample Type Serum, Plasma, Tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol DIAPH1
Gene id 1729
Alias Symbols DFNA1, DIA1, DIAP1, Diaphanous-related formin-1, DRF1, FLJ25265, hDIA1, LFHL1, Protein diaphanous homolog 1
Swissprot ID O60610
Protein Name protein diaphanous homolog 1
Description of Target This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphano
Kit Principle Genway Biotech DIAPH1 ELISA Kit (Human) (GWB-KBBSV3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DIAPH1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DIAPH1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DIAPH1 captured in the well.
Kit Component
ComponentAmount
Anti-DIAPH1 Microplate96 Wells (12 x 8 Well Strips)
DIAPH1 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X DIAPH1 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001073280.1
Nucleotide Accession Num NM_001079812.2
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBSV3
Species Reactivity Human