Dkk1 ELISA Kit (Rat) (GWB-KBBLW6) (Aviva Cat. No. OKCD01537)

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Please see item number OKCD01537 to view product details and for ordering information.


GWB-KBBLW6

Aviva Cat. No.: OKCD01537

Additional Information

Name Dkk1 ELISA Kit (Rat) (GWB-KBBLW6)
Kit Range 0.781 - 50 ng/mL
Sensitivity < 0.27 ng/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol DKK1
Gene id 293897
Alias Symbols Dkk1, Dickkopf homolog 1 (Xenopus laevis) (Predicted)
Swissprot ID D3Z9J1
Protein Name Dickkopf homolog 1 (Xenopus laevis) (Predicted)
Kit Principle Genway Biotech Dkk1 ELISA Kit (Rat) (GWB-KBBLW6) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Dkk1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Dkk1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Dkk1 captured in the well.
Kit Component
ComponentAmount
Anti-Dkk1 Microplate96 Wells (12 x 8 Well Strips)
Dkk1 Lyophilized Standard2 x 50 ng
100X Biotinylated Dkk1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001099820
Nucleotide Accession Num NM_001106350
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)80-95%82-101%80-92%92-105%
EDTA Plasma (n=5)83-104%89-103%89-96%85-97%
Heparin Plasma (n=5)98-105%88-96%78-99%98-105%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)82-9490
EDTA Plasma (n=5)87-9491
Heparin Plasma (n=5)78-10284
Datasheets/Manuals Printable datasheet for GWB-KBBLW6
Species Reactivity Rat