| Name |
DLAT ELISA Kit (Mouse) (GWB-KBBXA5) |
| Kit Range |
0.156-10 ng/mL |
| Sensitivity |
< 0.048 ng/mL |
| Sample Type |
Serum, Plasma, Tissue homogenates, Cell lysates |
| Kit Detection |
Absorbance, OD 450 nm |
| Gene Symbol |
DLAT |
| Gene id |
235339 |
| Alias Symbols |
6332404G05Rik, Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex, Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial, DLTA, PDCE2, PDC-E2, Pyruvate dehydrogenase complex co |
| Swissprot ID |
Q8BMF4 |
| Protein Name |
dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial |
| Description of Target |
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle. |
| Kit Principle |
Genway Biotech DLAT ELISA Kit (Mouse) (GWB-KBBXA5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DLAT has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DLAT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DLAT captured in the well. |
| Kit Component |
| Component | Amount |
| Anti-DLAT Microplate | 96 Wells (12 x 8 Well Strips) |
| DLAT Lyophlized Standard | 2 x 10 ng |
| Standard Diluent | 1 x 20 mL |
| 100X DLAT Biotinylated Detector Antibody | 1 x 120 µL |
| 100X Avidin-HRP Detection Conjugate | 1 x 120 µL |
| Detector Antibody Diluent | 1 x 12 mL |
| Detection Conjugate Diluent | 1 x 12 mL |
| 10X Sample Diluent | 1 x 30 mL |
| 30X Wash Buffer | 1 x 20 mL |
| TMB Substrate | 1 x 9 mL |
| Stop Solution | 1 x 6 mL | |
| Protein Accession Num |
NP_663589.3 |
| Nucleotide Accession Num |
NM_145614.4 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Kit Duration |
~ 3.5 Hours |
| Datasheets/Manuals |
Printable datasheet for GWB-KBBXA5 |
| Species Reactivity |
Mouse |
