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DLAT ELISA Kit (Mouse) (GWB-KBBXA5)

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GWB-KBBXA5

96 Wells
$659.00
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Additional Information

Name DLAT ELISA Kit (Mouse) (GWB-KBBXA5)
Description of Target The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle.
Kit Range 0.156-10 ng/mL
Sensitivity < 0.048 ng/mL
Sample Type Serum, Plasma, Tissue homogenates, Cell lysates
Kit Detection Absorbance, OD 450 nm
Gene Symbol DLAT
Gene id 235339
Alias Symbols 6332404G05Rik, Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex, Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial, DLTA, PDCE2, PDC-E2, Pyruvate dehydrogenase complex co
Swissprot ID Q8BMF4
Protein Name dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
Kit Principle Genway Biotech DLAT ELISA Kit (Mouse) (GWB-KBBXA5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DLAT has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DLAT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DLAT captured in the well.
Kit Component
ComponentAmount
Anti-DLAT Microplate96 Wells (12 x 8 Well Strips)
DLAT Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X DLAT Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_663589.3
Nucleotide Accession Num NM_145614.4
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBXA5
Species Reactivity Mouse