Dlk1 ELISA Kit (Rat) (GWB-KBBQF6) (Aviva Cat. No. OKCD01281)

This exact product is available to purchase through Aviva Systems Biology.
Please see item number OKCD01281 to view product details and for ordering information.


GWB-KBBQF6

Aviva Cat. No.: OKCD01281

Additional Information

Name Dlk1 ELISA Kit (Rat) (GWB-KBBQF6)
Kit Range 0.78 - 50 ng/mL
Sensitivity < 0.27 ng/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol DLK1
Gene id 114587
Alias Symbols Pref-1, Zog
Swissprot ID O70534
Protein Name protein delta homolog 1
Description of Target involved in maintaining the undifferentiated status of the preadipocyte [RGD, Feb 2006]
Kit Principle Genway Biotech Dlk1 ELISA Kit (Rat) (GWB-KBBQF6) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Dlk1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Dlk1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Dlk1 captured in the well.
Kit Component
ComponentAmount
Anti-Dlk1 Microplate96 Wells (12 x 8 Well Strips)
Dlk1 Lyophilized Standard2 x 50 ng
100X Biotinylated Dlk1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_446196
Nucleotide Accession Num NM_053744
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)95-105%93-102%98-105%79-95%
EDTA Plasma (n=5)82-93%85-96%97-105%80-98%
Heparin Plasma (n=5)83-91%98-105%95-102%97-104%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)81-9284
EDTA Plasma (n=5)85-9592
Heparin Plasma (n=5)98-105102
Datasheets/Manuals Printable datasheet for GWB-KBBQF6
Species Reactivity Rat