dLL4 ELISA Kit (Rat) (GWB-KBBXP5) (Aviva Cat. No. OKDD00764)

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Please see item number OKDD00764 to view product details and for ordering information.


GWB-KBBXP5

Aviva Cat. No.: OKDD00764

Additional Information

Name dLL4 ELISA Kit (Rat) (GWB-KBBXP5)
Kit Range 0.156-10 ng/mL
Sensitivity <0.068ng/mL
Sample Type Serum, Plasma, Tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol dLL4
Gene id 311332
Swissprot ID D3ZHH1
Protein Name delta-like protein 4
Description of Target Involved in the notch signaling pathway as notch ligand. activates notch1 and notch4 involved in angiogenesis, negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. essential for retinal progenitor proliferation. requ
Kit Principle Genway Biotech dLL4 ELISA Kit (Rat) (GWB-KBBXP5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for dLL4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for dLL4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample dLL4 captured in the well.
Kit Component
ComponentAmount
Anti-dLL4 Microplate96 Wells (12 x 8 Well Strips)
dLL4 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X dLL4 Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num XP_006234827.1
Nucleotide Accession Num XM_006234765.2
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBXP5
Species Reactivity Rat