| Name |
dLL4 ELISA Kit (Rat) (GWB-KBBXP5) |
| Kit Range |
0.156-10 ng/mL |
| Sensitivity |
<0.068ng/mL |
| Sample Type |
Serum, Plasma, Tissue homogenates |
| Kit Detection |
Absorbance, OD 450 nm |
| Gene Symbol |
dLL4 |
| Gene id |
311332 |
| Swissprot ID |
D3ZHH1 |
| Protein Name |
delta-like protein 4 |
| Description of Target |
Involved in the notch signaling pathway as notch ligand. activates notch1 and notch4 involved in angiogenesis, negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. essential for retinal progenitor proliferation. requ |
| Kit Principle |
Genway Biotech dLL4 ELISA Kit (Rat) (GWB-KBBXP5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for dLL4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for dLL4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample dLL4 captured in the well. |
| Kit Component |
| Component | Amount |
|---|
| Anti-dLL4 Microplate | 96 Wells (12 x 8 Well Strips) |
| dLL4 Lyophilized Standard | 2 x 10 ng |
| Standard Diluent | 1 x 20 mL |
| 100X dLL4 Biotinylated Detector Antibody | 1 x 120 uL |
| 100X Avidin-HRP Detection Conjugate | 1 x 120 uL |
| Detector Antibody Diluent | 1 x 12 mL |
| Detection Conjugate Diluent | 1 x 12 mL |
| 30X Wash Buffer | 1 x 20 mL |
| TMB Substrate | 1 x 9 mL |
| Stop Solution | 1 x 6 mL |
|
| Protein Accession Num |
XP_006234827.1 |
| Nucleotide Accession Num |
XM_006234765.2 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Kit Duration |
~ 3.5 Hours |
| Datasheets/Manuals |
Printable datasheet for GWB-KBBXP5 |
| Species Reactivity |
Rat |
