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96 Wells
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Additional Information

Name DNAJC4 ELISA Kit (Human) (GWB-KBBAF1)
Kit Range 0.781 - 50 ng/mL
Sensitivity < 0.27 ng/mL
Sample Type tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol DNAJC4
Gene id 3338
Alias Symbols DANJC4, DnaJ homolog subfamily C member 4, DnaJ-like protein HSPF2, HSPF2, MCG18, MGC19482, MGC57189, MGC71863, Multiple endocrine neoplasia type 1 candidate protein number 18
Swissprot ID Q9NNZ3
Protein Name dnaJ homolog subfamily C member 4
Kit Principle Genway Biotech DNAJC4 ELISA Kit (Human) (GWB-KBBAF1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DNAJC4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DNAJC4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DNAJC4 captured in the well.
Kit Component
Anti-RFK Microplate96 Wells (12 x 8 Well Strips)
RFK Lyophilized Standard2 x 50 ng
100X Biotinylated RFK Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001294909
Nucleotide Accession Num NM_001307980
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBAF1
Species Reactivity Human