DNASE1L1 ELISA Kit (Human) (GWB-KBBKV7) (Aviva Cat. No. OKCD00450)

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GWB-KBBKV7

Aviva Cat. No.: OKCD00450

Additional Information

Name DNASE1L1 ELISA Kit (Human) (GWB-KBBKV7)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.057 ng/mL
Sample Type tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol DNASE1L1
Gene id 1774
Alias Symbols Deoxyribonuclease-1-like 1, Deoxyribonuclease I-like 1, DNAS1L1, DNase I-like 1, DNASEX, DNase X, DNL1L, Muscle-specific DNase I-like, XIB
Swissprot ID P49184
Protein Name deoxyribonuclease-1-like 1
Description of Target This gene encodes a deoxyribonuclease protein that shows high sequence similarity to DNase I. The encoded protein is localized to the endoplasmic reticulum and modified by N-linked glycosylation. Alternate transcriptional splice variants encoding the same
Kit Principle Genway Biotech DNASE1L1 ELISA Kit (Human) (GWB-KBBKV7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DNASE1L1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DNASE1L1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DNASE1L1 captured in the well.
Kit Component
ComponentAmount
Anti-SNX17 Microplate96 Wells (12 x 8 Well Strips)
SNX17 Lyophilized Standard2 x 10 ng
100X Biotinylated SNX17 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001009932
Nucleotide Accession Num NM_001009932
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBKV7
Species Reactivity Human