DNASEX ELISA Kit (Human) (GWB-KBBFF6)

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GWB-KBBFF6

96 Wells
$779.00

Additional Information

Name DNASEX ELISA Kit (Human) (GWB-KBBFF6)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.057 ng/mL
Sample Type Serum, Plasma, Tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol DNASEX
Gene id 1774
Alias Symbols Deoxyribonuclease-1-like 1, Deoxyribonuclease I-like 1, DNAS1L1, DNase I-like 1, DNASEX, DNase X, DNL1L, Muscle-specific DNase I-like, XIB
Swissprot ID P49184
Protein Name Deoxyribonuclease-1-like 1
Description of Target This gene encodes a deoxyribonuclease protein that shows high sequence similarity to DNase I. The encoded protein is localized to the endoplasmic reticulum and modified by N-linked glycosylation. Alternate transcriptional splice variants encoding the same
Kit Principle Genway Biotech DNASEX ELISA Kit (Human) (GWB-KBBFF6) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DNASEX has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DNASEX is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DNASEX captured in the well.
Kit Component
ComponentAmount
Anti-DNASEX Microplate96 Wells (12 x 8 Well Strips)
DNASEX Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X DNASEX Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001009932.1
Nucleotide Accession Num NM_001009932.2
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBFF6
Species Reactivity Human