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96 Wells
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Additional Information

Name DNMT3B ELISA Kit (Human) (GWB-KBBAD4)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.054 ng/mL
Sample Type Serum, plasma, tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol DNMT3B
Gene id 1789
Alias Symbols DNA (cytosine-5)-methyltransferase 3B, DNA methyltransferase HsaIIIB, DNA MTase HsaIIIB, Dnmt3b, ICF, M.HsaIIIB
Swissprot ID Q9UBC3
Protein Name DNA (cytosine-5)-methyltransferase 3B
Description of Target CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA met
Kit Principle Genway Biotech DNMT3B ELISA Kit (Human) (GWB-KBBAD4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DNMT3B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DNMT3B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DNMT3B captured in the well.
Kit Component
Anti-DNMT3B Microplate96 Wells (12 x 8 Well Strips)
DNMT3B Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X DNMT3B Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001193984.1
Nucleotide Accession Num NM_001207055.1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBAD4
Species Reactivity Human