DOCK4 ELISA Kit (Human) (GWB-KBBJR7)

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GWB-KBBJR7

96 Wells
$659.00
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Additional Information

Name DOCK4 ELISA Kit (Human) (GWB-KBBJR7)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.063 ng/mL
Sample Type tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol DOCK4
Gene id 9732
Alias Symbols Dedicator of cytokinesis protein 4, FLJ34238, KIAA0716, MGC134911, MGC134912
Swissprot ID Q8N1I0
Protein Name dedicator of cytokinesis protein 4
Description of Target Involved in regulation of adherens junction between cells. Plays a role in cell migration. Functions as a guanine nucleotide exchange factor (GEF), which activates Rap1 small GTPase by exchanging bound GDP for free GTP.
Kit Principle Genway Biotech DOCK4 ELISA Kit (Human) (GWB-KBBJR7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DOCK4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DOCK4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DOCK4 captured in the well.
Kit Component
ComponentAmount
Anti-CLSTN2 Microplate96 Wells (12 x 8 Well Strips)
CLSTN2 Lyophilized Standard2 x 10 ng
100X Biotinylated CLSTN2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_055520
Nucleotide Accession Num NM_014705
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBJR7
Species Reactivity Human