Canine Albumin ELISA Kit (GWB-1BB207) (Aviva Cat. No. OKIA00021)

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Please see item number OKIA00021 to view product details and for ordering information.


GWB-1BB207

Aviva Cat. No.: OKIA00021

40-374-130024

Description

Short Description: In this assay the albumin present in serum sample reacts with the anti-Alb. antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound serum proteins by washing, anti-Alb. antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound serum Alb. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Albumin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Albumin in the test sample. The quantity of Albumin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for serum dilution.

Additional Information

Name Canine Albumin ELISA Kit (GWB-1BB207)
Related Product Names Dog Albumin ELISA Kit; Allergen Can f 3 Dog AlbuminALB
Kit Range 12.5 ng/ml - 400 ng/ml
Sensitivity 2.463 ng/ml
Sample Type Serum, plasma and other biological fluids
Gene id 403550
Alias Symbols Alpha fetoglobulin
Swissprot ID P49822
Protein Name Serum albumin
Description of Target Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma,
Kit Principle Genway Biotech Albumin ELISA Kit (Dog) (GWB-1BB207) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Serum albumin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Serum albumin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Serum albumin captured in the well.
Kit Component
ComponentAmount
Anti-Dog Albumin Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Dog Albumin Calibrator1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Homology Dog
Standard Range Refer To Manual
Product Format In Solution
Specimen/Volumes Refer To Manual
Application ELISA
Reconstitution and Storage 1. DILUENT
The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.
2. WASH SOLUTION
The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.
3. ENZYME-ANTIBODY CONJUGATE
Undiluted horseradish peroxidase anti-Albumin conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.
4. CHROMOGEN-SUBSTRATE SOLUTION
The Substrate Solution should be stored at 4-8C and is stable until the expiration date.
5. STOP SOLUTION
The Stop Solution should be stored at 4-8C and is stable until the expiration date.
6. ANTI-DOG ALBUMIN ELISA MICRO PLATE
Anti-Dog Albumin coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.
7. DOG ALBUMIN CALIBRATOR
The lyophilized Dog Albumin calibrator should be stored at 4C or frozen until reconstituted. The reconstituted calibrator should be aliquoted out and stored frozen (Avoid multiple freeze-thaw cycles). The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours.
Reactivity Canine
Kit Size 96 well
Kit Duration 100 Minutes
Datasheets/Manuals Printable datasheet for GWB-1BB207
Storage Refer to kit instructions for component storage
Species Reactivity Dog
Lead Time Domestic: within 1 week delivery | International: 1 week
Intended Use Research Use Only
Additional Information Intended Use: The Albumin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Albumin in biological fluid of Dogs.
:: Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Dog Albumin antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-DOG ALBUMIN ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Dog Albumin.
7. DOG ALBUMIN CALIBRATOR
One vial containing a lyophilized Dog Albumin calibrator.