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96 Wells
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Additional Information

Name DPEP2 ELISA Kit (Human) (GWB-KBBUN4)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.069 ng/mL
Sample Type Serum, plasma, tissue homogenates, cell lysates, cell culture supernatant.
Kit Detection Absorbance, OD 450 nm
Gene Symbol DPEP2
Gene id 64174
Alias Symbols Dipeptidase 2, UNQ284/PRO323
Swissprot ID Q9H4A9
Protein Name Dipeptidase 2
Description of Target DPEP2 belongs to the membrane-bound dipeptidase (EC family. These enzymes hydrolyze a variety of dipeptides, including leukotriene D4, the beta-lactam ring of some antibiotics, and cystinyl-bis-glycine (cys-bis-gly) formed during glutathione de
Kit Principle Genway Biotech DPEP2 ELISA Kit (Human) (GWB-KBBUN4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DPEP2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DPEP2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DPEP2 captured in the well.
Kit Component
Anti-DPEP2 Microplate96 Wells (12 x 8 Well Strips)
DPEP2 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X DPEP2 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_071750.1
Nucleotide Accession Num NM_022355.3
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBUN4
Species Reactivity Human