DPEP3 ELISA Kit (Human) (GWB-KBBRU4) (Aviva Cat. No. OKCD00973)

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Please see item number OKCD00973 to view product details and for ordering information.


Aviva Cat. No.: OKCD00973

Additional Information

Name DPEP3 ELISA Kit (Human) (GWB-KBBRU4)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.105 ng/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol DPEP3
Gene id 64180
Alias Symbols Dipeptidase 3, UNQ834/PRO1772
Swissprot ID Q9H4B8
Protein Name dipeptidase 3
Description of Target Probable metalloprotease which hydrolyzes cystinyl-bis-glycine. May be involved in meiosis (
Kit Principle Genway Biotech DPEP3 ELISA Kit (Human) (GWB-KBBRU4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DPEP3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DPEP3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DPEP3 captured in the well.
Kit Component
Anti-KIF14 Microplate96 Wells (12 x 8 Well Strips)
KIF14 Lyophilized Standard2 x 20 ng
100X Biotinylated KIF14 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001123230
Nucleotide Accession Num NM_001129758
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)91-99%90-104%80-90%98-105%
EDTA Plasma (n=5)91-99%79-96%82-91%85-93%
Heparin Plasma (n=5)96-103%89-101%79-94%89-103%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)98-105101
EDTA Plasma (n=5)80-9285
Heparin Plasma (n=5)88-10195
Datasheets/Manuals Printable datasheet for GWB-KBBRU4
Species Reactivity Human