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96 Wells
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Additional Information

Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.126 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol DROSHA
Gene id 29102
Alias Symbols Etohi2, ETOHI2, HSA242976, p241, Protein Drosha, RANSE3L, Ribonuclease 3, Ribonuclease III, RN3, RNASE3L, RNase III, RNASEN
Swissprot ID Q9NRR4
Protein Name ribonuclease 3
Description of Target Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release
Kit Principle Genway Biotech DROSHA ELISA Kit (Human) (GWB-KBBJI8) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DROSHA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DROSHA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DROSHA captured in the well.
Kit Component
Anti-Lipa Microplate96 Wells (12 x 8 Well Strips)
Lipa Lyophilized Standard2 x 20 ng
100X Biotinylated Lipa Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001093882
Nucleotide Accession Num NM_001100412
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBJI8
Species Reactivity Human