DSC1 ELISA Kit (Human) (GWB-KBBEK3)

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GWB-KBBEK3

96 Wells
$659.00
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Additional Information

Name DSC1 ELISA Kit (Human) (GWB-KBBEK3)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.059 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol DSC1
Gene id 1823
Alias Symbols Cadherin family member 1, CDHF1, Desmocollin-1, Desmosomal glycoprotein 2/3, DG2/DG3
Swissprot ID Q08554
Protein Name desmocollin-1
Description of Target Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. May contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness be
Kit Principle Genway Biotech DSC1 ELISA Kit (Human) (GWB-KBBEK3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DSC1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DSC1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DSC1 captured in the well.
Kit Component
ComponentAmount
Anti-DSC2 Microplate96 Wells (12 x 8 Well Strips)
DSC2 Lyophilized Standard2 x 10 ng
100X Biotinylated DSC2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_004939
Nucleotide Accession Num NM_004948
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBEK3
Species Reactivity Human