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96 Wells
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Additional Information

Name DTNB ELISA Kit (Human) (GWB-KBBZW8)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.053 ng/mL
Sample Type tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol DTNB
Gene id 1838
Alias Symbols Beta-dystrobrevin, DTN-B, Dystrobrevin beta, MGC17163, MGC57126
Swissprot ID O60941
Protein Name dystrobrevin beta
Description of Target This gene encodes dystrobrevin beta, a component of the dystrophin-associated protein complex (DPC). The DPC consists of dystrophin and several integral and peripheral membrane proteins, including dystroglycans, sarcoglycans, syntrophins and dystrobrevin
Kit Principle Genway Biotech DTNB ELISA Kit (Human) (GWB-KBBZW8) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DTNB has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DTNB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DTNB captured in the well.
Kit Component
Anti-Spon2 Microplate96 Wells (12 x 8 Well Strips)
Spon2 Lyophilized Standard2 x 10 ng
100X Biotinylated Spon2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001243232
Nucleotide Accession Num NM_001256303
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBZW8
Species Reactivity Human