| Name |
DYNC1H1 ELISA Kit (Human) (GWB-KBBLF4) |
| Kit Range |
0.312 - 20 ng/mL |
| Sensitivity |
< 0.113 ng/mL |
| Sample Type |
tissue homogenates and other biological fluids. |
| Kit Detection |
Colorimetric, OD 450 nm |
| Gene Symbol |
DYNC1H1 |
| Gene id |
1778 |
| Alias Symbols |
Cytoplasmic dynein 1 heavy chain 1, Cytoplasmic dynein heavy chain 1, DHC1, DHC1a, DKFZp686P2245, DNCH1, Dnchc1, DNCL, DNECL, DYHC, Dynein heavy chain, cytosolic, HL-3, KIAA0325, p22 |
| Swissprot ID |
Q14204 |
| Protein Name |
cytoplasmic dynein 1 heavy chain 1 |
| Description of Target |
Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. |
| Kit Principle |
Genway Biotech DYNC1H1 ELISA Kit (Human) (GWB-KBBLF4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DYNC1H1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DYNC1H1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DYNC1H1 captured in the well. |
| Kit Component |
| Component | Amount |
|---|
| Anti-UGGT1 Microplate | 96 Wells (12 x 8 Well Strips) | | UGGT1 Lyophilized Standard | 2 x 20 ng | | 100X Biotinylated UGGT1 Detector Antibody | 120 uL | | Avidin/HRP Conjugate | 120 uL | | Standard Diluent | 1 x 20 mL | | Detector Antibody Diluent | 1 x 12 mL | | Conjugate Diluent | 1 x 12 mL | | 30X Wash Buffer | 1 x 20 mL | | TMB Substrate | 1 x 9 mL | | Stop Solution | 1 x 6 mL |
|
| Protein Accession Num |
NP_001367 |
| Nucleotide Accession Num |
NM_001376 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Kit Duration |
~ 3 Hours |
| Datasheets/Manuals |
Printable datasheet for GWB-KBBLF4 |
| Species Reactivity |
Human |
