DYNC1H1 ELISA Kit (Human) (GWB-KBBLF4) (Aviva Cat. No. OKCD01052)

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Aviva Cat. No.: OKCD01052

Additional Information

Name DYNC1H1 ELISA Kit (Human) (GWB-KBBLF4)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.113 ng/mL
Sample Type tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol DYNC1H1
Gene id 1778
Alias Symbols Cytoplasmic dynein 1 heavy chain 1, Cytoplasmic dynein heavy chain 1, DHC1, DHC1a, DKFZp686P2245, DNCH1, Dnchc1, DNCL, DNECL, DYHC, Dynein heavy chain, cytosolic, HL-3, KIAA0325, p22
Swissprot ID Q14204
Protein Name cytoplasmic dynein 1 heavy chain 1
Description of Target Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP.
Kit Principle Genway Biotech DYNC1H1 ELISA Kit (Human) (GWB-KBBLF4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DYNC1H1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DYNC1H1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DYNC1H1 captured in the well.
Kit Component
Anti-UGGT1 Microplate96 Wells (12 x 8 Well Strips)
UGGT1 Lyophilized Standard2 x 20 ng
100X Biotinylated UGGT1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001367
Nucleotide Accession Num NM_001376
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBLF4
Species Reactivity Human