Ear3 ELISA Kit (Mouse) (GWB-KBBUD7) (Aviva Cat. No. OKCD01136)

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Please see item number OKCD01136 to view product details and for ordering information.


GWB-KBBUD7

Aviva Cat. No.: OKCD01136

Additional Information

Name Ear3 ELISA Kit (Mouse) (GWB-KBBUD7)
Kit Range 62.5 - 4,000 pg/mL
Sensitivity < 28.1 pg/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol EAR3
Gene id 53876
Alias Symbols Eosinophil cationic-type ribonuclease 3, mR3, MR-3, murine ribonuclease 3, Rnase3
Swissprot ID O35290
Protein Name eosinophil cationic-type ribonuclease 3
Kit Principle Genway Biotech Ear3 ELISA Kit (Mouse) (GWB-KBBUD7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ear3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ear3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ear3 captured in the well.
Kit Component
ComponentAmount
Anti-Cpn1 Microplate96 Wells (12 x 8 Well Strips)
Cpn1 Lyophilized Standard2 x 4 ng
100X Biotinylated Cpn1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_059084
Nucleotide Accession Num NM_017388
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)79-98%80-89%94-103%87-98%
EDTA Plasma (n=5)97-105%88-96%94-101%80-92%
Heparin Plasma (n=5)85-95%90-98%83-98%78-101%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)80-9781
EDTA Plasma (n=5)78-10396
Heparin Plasma (n=5)90-10196
Datasheets/Manuals Printable datasheet for GWB-KBBUD7
Species Reactivity Mouse