Alkaline Phosphatase Conjugated Affinity Purified Anti-RABBIT IgG F(ab') 2 (GOAT) (GWB-352F7B) (Aviva Cat. No. OARA04670)

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GWB-352F7B

Aviva Cat. No.: OARA04670

18-732-292606

Additional Information

Name Alkaline Phosphatase Conjugated Affinity Purified Anti-RABBIT IgG F(ab') 2 (GOAT) (GWB-352F7B)
Related Product Names Alkaline Phosphatase Conjugated Affinity Purified anti-Rabbit IgG F(ab’)2 [Goat]; EC 3.1.3.1; AP-TNAP; TNSALP; Alkaline phosphatase liver/bone/kidney isozyme Alkaline Phosphatase Conjugated Affinity Purified Anti-RABBIT IgG F(ab\')2 (GOAT)ALPL
Alias Symbols ALP, Alk Phos
Swissprot ID P05186
Description of Target Anti-Rabbit IgG F(ab')2 Antibody generated in goat recognizes the dimeric Fab portion of the rabbit IgG molecule. Rabbit IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH. F(ab')2 molecules lack the Fc portion of IgG and therefore receptors that bind rabbit IgG F(c) will not bind rabbit IgG F(ab')2 molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Clone Polyclonal
Application Info ELISA: 1:2000~10000
IHC: 1:200~1000
WB: 1:500~2500
Host Goat
Immunogen Rabbit IgG F(ab')2 fragment
Conjugation Alkaline Phosphatase (AP)
NCBI Acc Number NP_000469.3
Product Format Liquid 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol (pH 8.0), 10 mg/ml BSA (Immunoglobulin & Protease free) with 0.01% (w/v) sodium azide
Molecular Weight 140 kDa
Purification This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab’)2 and Rabbit Serum. No reaction was observed against Rabbit IgG F(c).
Concentration 0.75 mg/ml (UV absorbance at 280nm)
Application WB, ELISA, IHC. This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.
Stability Expiration date is one (1) year from date of opening.
Reconstitution and Storage Store vial at 4°C before opening. DO NOT FREEZE. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Reactivity Rabbit IgG F(ab\')2
Datasheets/Manuals Printable datasheet for GWB-352F7B
Lead Time Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Intended Use Research Use Only
Additional Information Conjugation Chemistry: Glutaraldehyde coupling
Color: Substrate dependent