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Product Name: Anti-TG

# of Samples: 1 x 96 Assays

Intended Use: Immunoenzymatic colorimetric method (ELISA) for quantitative determination of Anti-TG in serum or plasma.

Introduction: Thyroglobulin (TG) is a protein produced by and used entirely within the thyroid gland. Thyroglobulin is used by the thyroid gland to produce the thyroid hormones thyroxine (T4) and triiodothyronine (T3). Thyroglobulin synthesis is stimulated at the transcriptional level by thyroid-stimulating hormone (TSH)
Thyroglobulin (TG) is a well-known target for autoantibodies occurring in thyroid autoimmunity (Graves\' disease and Hashimoto\'s thyroiditis). Anti-TG antibodies mostly belong to the IgG class. Low to moderate levels of anti-TG antibodies can be found in sera of other autoimmune patients (eg systemic lupus erythematosus or Sjogren syndrome). In some cases anti-TG positive sera may show negativity for other type of anti-thyroid antibodies - anti-TPO. Therefore, combined determination of both types of anti-thyroid antibodies (anti-TPO + anti-TG) provides most sensitive laboratory diagnostic tool for thyroid autoimmunity. Separately from autoimmunity, anti-TG antibodies may develop in patients suffering from thyroid cancer. High level of anti-TG in such patients may interfere with correct determination of serum thyroglobulin, which serves as tumour marker for therapy control in this group of patients.

Principles of the assay: This test is based on two-site sandwich enzyme immunoassay principle. The mircoplates are coated with Thyroglobulin. In the first step antibodies from the sample bind to the coated antigen on the microwell surface. Unbound material is removed by a washing procedure. Second antibodies directed against human IgG and labelled with horseradish peroxidase, are then added to the microwells. A second washing step is performed. Then the immune complex is visualized by adding Tetramethylbenzidine (TMB) substrate, which gives a blue reaction product. The intensity of this product is proportional to the amount of Anti-TG in samples and standards. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorption at 450 nm is read using an ELISA microwell plate reader.

Storage and Stability: The reagents are stable up to the expiry date stated on the label when stored at 2...8 °C in the original container.

Limitations of the Test: Bacterial contamination or repeated freeze-thaw cycles of the specimen may affect the absorbance values.

References Volpè R. (1990) Autoimmune disease in endocrine system; Boca Raton FL; CRC Press.Volpè R. (1994) Clin. Chem. 40, 2132 - 2145.Beever K. (1989) Clin. Chem. 35, 1949-54.Mak T.W. (1994) Clin. Chem. 40, 2128 - 2131.Chiavato L.and Pinchera A. (1991) Autoimmunity 10, 319-31.Degroot L.J., Thyroperoxidase Thyroid Autoimmunity 207:177 – 182 (1990)

Additional Information

Name Anti-TG
Related Product Names Anti-TG
Storage The reagents are stable up to the expiry date stated on the label when stored at 2...8 °C in the original container.
Datasheets/Manuals Printable manual for GWB-191310
Intended Use Research Use Only