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ApopLadder Ex

GWB-211D09

40-831-160020

ELISA_Kits
$219.30
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Description

Products Name: ApopLadder Ex

Introduction: One of the features of apoptotic cells is fragmentation of chromatin DNA at nucleosome level (185 bp). This fragmented DNA is usually detected with the laddering in gel electrophoresis. ApopLadder Ex is a kit which consists of Lysis buffer, including a unique surfactant, enzymes, precipitant of DNA and Loading buffer. This Kit enables the selective extraction of fragmented DNA and highly sensitive detection of the laddering DNA by minimizing contamination of the intact chromatin. This kit is also useful for quantitative determination using a fluorescent dye.

Principle of the test: In this kit, DNA freagments can be separated from intact chromatin DNA by treatment of apoptotic cells with lysis buffer, centrifugation and recovery of its supernatant. After degrading impurities (e.g., proteins, RNAs) by enzymes, DNA fragments can be recovered by precipitant.

Kit Components: (For 24 assays)
[1] Lysis buffer.......................................................................... 4 x 1.2 ml
[2] 10% SDS solution................................................................. 4 x 0.48 ml
[3] Enzyme A ............................................................................. 1 x 0.48 ml
[4] Enzyme B ............................................................................. 1 x 0.48 ml
[5] Precipitant ............................................................................ 3 x 1.04 ml
[6] 6 x Loading buffer ............................................................... 1 x 0.48 ml

Product Protocol: To see this product used in a peer reviewed journal, click the following link:
Soy-Derived Isoflavones Inhibit the Growth of Canine Lymphoid Cell Lines

Additional Information

Name ApopLadder Ex
Related Product Names ApopLadder Ex ELISA Kit ApopLadder Ex
Datasheets / Downloads GWB-211D09 Datasheet
Intended Use Research Use Only