GPDH Activity Assay Kit





Product Name GPDH Activity Assay Kit.
Description Biosynthesis of fatty acids, one of the processes of carbon dioxide fixation in the living organisms, takes place by using acetyl-Cox as a direct starting source. Among the synthesized fatty acids, the long-chain fatty acids in particular have surface-activity and cytotoxicity in the free state.Therefore they are not allowed to accumulate in the cells. To avoid this accumulation, the long-chain fatty acids rapidly form esters with glycerol 3-phosphate to generate triacylglycerols(fat) when a large amount of long-chain fatty acids are synthesized in the organisms. In the liver, glycerol 3-phosphate is generated by di rect phosphorylation of glycerol, while in the muscles and adiopocytes, it is generated by reducing dihydroxyacetone phosphate, an intermediate product in the glycolytic pathway, with NADH (Fig. 1). Accordingly, lip id synthesis does not occur without glucose in the muscles and adipocytes even if there exist a highest concentration of fatty acids.
Enzyme which catalyzes the reversible conversion between dihydroxyacetone phosphate and glycerol 3-phophate using NAD as a coenzyme is called glycerol 3-phosphate dehydrogenase: GPDH : NAD' 2 - oxidoreductase (EC1.1.1.8) and regarded as a representative marker for adipocyte differentiation from the progenitor cells. In adipocytes and muscles, glycerol 3-phosphate dehydrogenase : NAD' 2 oxidoreductase (EC1.1.1.8)is found in the cytosol and another glycerol3-phosphate dehydrogenase: (acceptor) oxidoreductase (EC using FADas a coenzyme is responsible fo r th e reverse reaction of glycerol3-phosphate to dihydroxyacetone phosphate in the mitochondria. These two enzymes exchange the substrates for the reactions to form the glycerol phosphate cycle.
GenWay's Glycerol3-phophate dehydrogenase (GPDH) activity assay kit is designed to determine the activities of GPDH (EC1.1.1.8), an enzyme that generates glycerol 3-phophate from dihydroxyacetone phosphate using NAD as a coenzyme, in the samples extracted from cells. The GPDH activity is known to rapidly increase on differentiation of adipocyte progenitor cells into adipocytes. ln the experimental systems, in which established lines of or prim aryadipocyteprogenitor cells are differentiated , the GPDH activity has been used as a major indicator of the differentiation for screening of the differentiation inhibitors or for elucidating the mechanism of the differentiation. This kit is designed for assays using 96-we ll plates, which enables determination of a large number of samples at a time. This product allows sample preparation and determination to be completed in a short time, which reduces the risk of enzyme inactivation and ensures acquisition of reproducible data.
# of Samples 1X96 Assays.
Storage and Stability -20°C or below.
Product Name GPDH Activity Assay Kit.
Catalytic Activity sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.
Enzyme Regulation Inhibited by zinc ions and sulfate.
Subunit Homodimer.
Subcellular Location Cytoplasm.
Similarity Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.

Additional Information

Name GPDH Activity Assay Kit
Related Product Names GPDH Activity Assay Kit ELISA Kit; GPDH-C; GPD-C; EC GPDH Activity Assay KitGPD1
Swissprot ID P21695
NCBI Acc Number NP_005267.2
Molecular Weight 37568
Datasheets/Manuals Printable datasheet for GWB-712ACB
Intended Use Research Use Only