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Human Myeloperoxidase ELISAKit

GWB-2795F0

40-374-130042

96 Wells
$335.00
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Description

Short Description: In this assay the Myeloperoxidase present in serum sample reacts with the anti-MPO antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound serum proteins by washing, anti-MPO antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound serum MPO. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Myeloperoxidase in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Myeloperoxidase in the test sample. The quantity of Myeloperoxidase in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for serum dilution.

Additional Information

Name Human Myeloperoxidase ELISAKit
Related Product Names Human Myeloperoxidase ELISA Kit; MPO; EC 1.11.1.7 Human MyeloperoxidaseMPO
Drywet Surcharge 0
Gene Symbol MPO
Gene Fullname myeloperoxidase
Gene id 4353
Alias Symbols MPO
Protein Name Myeloperoxidase
Description of Target Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiol
Swissprot ID P05164
Species Reactivity Human
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Sensitivity 0.994 ng/ml
Kit Range 1.875 ng/ml - 120 ng/ml
Homology Human
NCBI Acc Number NP_000241.1
Standard Range Refer To Manual
Sample Type Serum, plasma and other biological fluids
Product Format In Solution
Datasheets / Downloads GWB-2795F0 Datasheet
Specimen/Volumes Refer To Manual
Swiss Prot Number P05164
Applications ELISA
Reconstitution and Storage 1. DILUENT
The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.
2. WASH SOLUTION
The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.
3. ENZYME-ANTIBODY CONJUGATE
Undiluted horseradish peroxidase anti-MPO conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.
4. CHROMOGEN-SUBSTRATE SOLUTION
The Substrate Solution should be stored at 4-8C and is stable until the expiration date.
5. STOP SOLUTION
The Stop Solution should be stored at 4-8C and is stable until the expiration date.
6. ANTI-HUMAN MYELOPEROXIDASE ELISA MICRO PLATE
Anti-Human MPO coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.
7. HUMAN MYELOPEROXIDASE CALIBRATOR
The lyophilized Human Myeloperoxidase calibrator should be stored at 4C or frozen until reconstituted. The reconstituted calibrator should be aliquoted out and stored frozen (Avoid multiple freeze-thaw cycles). The working standard solutions should be prepared immediately prior to use and are stable for up to 1 hours.
Reactivity Human
Kit Size 96 well
Kit Principle Genway Biotech Myeloperoxidase ELISA Kit (Human) (GWB-2795F0) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Myeloperoxidase has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Myeloperoxidase - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Myeloperoxidase captured in the well.
Kit Component
ComponentAmount
Anti-Human Myeloperoxidase Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Human Myeloperoxidase Calibrator1
Kit Duration 90 Minutes
Storage Refer to kit instructions for component storage
Intended Use Research Use Only
Additional Information Intended Use: The Myeloperoxidase test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Myeloperoxidase in serum, plasma of Human.
:: Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Human Myeloperoxidase antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-HUMAN MYELOPEROXIDASE ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human MPO.
7. HUMAN MYELOPEROXIDASE CALIBRATOR
One vial containing a lyophilized Human Myeloperoxidase calibrator.