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LDH Cytotoxicity Detection Kit



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Products Name: LDH Cytotoxicity Detection Kit Manual

Introduction: Cell death is assayed by the quantification of plasma membrane damage. Several standards methods for quantification of cellular viability were developed from the need for the sensitive, reliable and automated methods for precise determination of cell death. GenWay\'s LDH Cytotoxicity Detection Kit allows simple measurement of LDH activity released from the of damaged cells into the supernatant.

Principle of the test: This kit is designed to allow a precise, fast and simple colorimetric assay method to quantitate cytotoxicity/cytolysis based on the measurement of LDH activity released from damaged cells into the supernatant. This is a non-radioactive alternative assay method to the [3H]-thymidine release assay and the [51Cr]-release assay. The culture supernatant is collected cell-free and incubated with the reaction mixture from the kit, and the LDH activity is determined in an enzymatic test as below: In the first step NAD+ is reduced to NADH/H+ by the LDH-catalyzed conversion of lactate to pyruvate. In the second step, the catalyst (diaphorase) transfers H/H+ from NADH/H+ to the tetrazolium salt INT which is reduced to formazan. An increase in the number of dead or plasma membrane-damaged cells leads to an increase of the LDH enzyme activity in the culture supernatant. This increase of the enzyme activity in the supernatant directly correlates to the amount of formazan formed in a certain time period. That is, the amount of color formed in the assay is proportional to the number of damaged cells. The formazan dye formed is water soluble and shows the maximum absorption at about 500 nm, whereas the tetrazolium salt INT shows no significant absorption at these wave lengths.

Kit Components: 2000 tests
- Bottle 1(blue cap) x 5: Catalyst (diaphorase/ NAD+, lyophilisate)
- Bottle 2(red cap) x 5: Dye solution (45 ml)
(containing iodotetrazolium chloride(INT) and sodium lactate)

Additional Information

Name LDH Cytotoxicity Detection Kit
Related Product Names LDH Cytotoxicity Detection Kit ELISA Kit LDH Cytotoxicity Detection Kit
Datasheets/Manuals Printable datasheet for GWB-787DA8
Intended Use Research Use Only