Caspase-3 Colorimetric Assay Kit - 100 assays

GWB-AXR017

100 assays
$415.80

Description

Description: Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-3/CPP32 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.

Summary:

- Detection method- Absorbance (400 or 405 nm)

- Sample type- Cell and tissue lysates

- Species reactivity- Mammalian

- Kit size- Convenient sizes (25, 100, 200, 400 assays)

- Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Kit components:

- Cell Lysis buffer

- 2X Reaction Buffer

- DEVD-pNA (4 mM)

- DTT (1 M)

- Dilution Buffer

Features and Benefits:

- Simple one-step procedure; takes 1-2 hours

- Fast and convenient

- Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.

Shipping Conditions: Gel pack

Additional Information

Name Caspase-3 Colorimetric Assay Kit - 100 assays
Related Product Names caspase3, caspase 3, Caspase-3 Colorimetric Assay Kit, Caspase-3, Caspase-3 Substrate, DVED, DEVD-pNA, Caspase-3 assay, Caspase-3 Colorimetric Assay, caspase-3 assay
Format Assay Kit
Storage -20C
Datasheets/Manuals Printable datasheet for GWB-AXR017
Intended Use Research Use Only