Caspase-3 Fluorometric Assay Kit - 25 assays (GWB-AXR015)

GWB-AXR015

25 assays
$176.40

Description

Description: Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The CPP32/Caspase-3 Fluorometric Protease Assay Kit provides a simple and convenient means for assaying the DEVD-dependent caspase activity. The assay is based on detection of cleavage of substrate DEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). DEVD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by CPP32 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3/CPP32 activity.

Summary:

- Detection method- Flow cytometry (400 nm excitation filter and 505 nm emission filter) and fluorescence microscopy

- Sample type- Cell and tissue lysates

- Species reactivity- Mammalian

- Kit size- Convenient sizes (25, 100, 200, 400 assays)

- Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Kit components:

- Cell Lysis Buffer

- 2X Reaction Buffer

- DEVD-AFC (1 mM)

- DTT (1 M)

Features and Benefits:

- Simple one-step procedure; takes 1-2 hours

- Fast and convenient

- Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3/CPP32 activity

Shipping Conditions: Gel pack

Additional Information

Name Caspase-3 Fluorometric Assay Kit - 25 assays (GWB-AXR015)
Availability Discontinued
Related Product Names caspase3, caspase 3, Caspase-3 Fluorometric Assay Kit, Caspase-3, Caspase-3 Substrate, DVED, DEVD-AFC, Caspase-3 assay, Caspase-3 Fluorometric Assay, caspase-3 assay, DEVDase
Format Assay Kit
Storage -20C
Datasheets/Manuals Printable datasheet for GWB-AXR015
Intended Use Research Use Only