Nitric Oxide Colorimetric Assay Kit

GWB-AXR175

200 assays
$472.15
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Description

Description: Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. NO is rapidly oxidized to nitrite and nitrate which are used to quantitate NO production. GenWay's Nitric Oxide Colorimetric Assay Kit provides an accurate, convenient measure of total nitrate/nitrite in a simple two-step process. The first step converts nitrate to nitrite utilizing nitrate reductase. The second step uses Griess Reagents to convert nitrite to a deep purple azo compound. The amount of the azochromophore accurately reflects nitric oxide amount in samples. The detection limit of the assay is approximately 0.1 nmole nitrite/well, or 1 µM.

Summary:

- Detection method- Absorbance (540 nm)

- Sample type- Cell and Tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids

- Species reactivity- Mammalian

- Applications- GenWay's Nitric Oxide Colorimetric Assay Kit provides an accurate, convenient measure of total nitrate/nitrite in a simple two-step process.

Kit components:

- Assay Buffer

- Enzyme cofactor

- Enhancer

- Nitrate Reductase

- Nitrate Standard

- Nitrite Standard

- Griess Reagent R1

- Griess Reagent R2

- Microtiter Plate

- Plate Cover

Features and Benefits:

- Simple two-step procedure; takes only ~1-1.5 hours

- Fast and convenient

- GenWay's Nitric Oxide Colorimetric Assay Kit provides an accurate, convenient measure of total nitrate/nitrite.

Shipping Conditions: Gel pack

Additional Information

Name Nitric Oxide Colorimetric Assay Kit
Related Product Names Nitric Oxide Colorimetric Assay Kit, Nitric Oxide assay, Nitric Oxide colorimetric assay, NO assay kit
Datasheets / Downloads GWB-AXR175 Datasheet
Format Assay Kit
Storage -20C
Intended Use Research Use Only