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LDH-Cytotoxicity Assay Kit

GWB-AXR194

400 assays
$332.10
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Description

Description: Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. The LDH-Cytotoxicity Assay Kit provides a fast and simple method for quantitating cytotoxicity based on the measurement of activity of lactate dehydrogenase (LDH) released from damaged cells. Unlike many other cytoplasmic enzymes which exist in many cells either in low amount (e.g., alkaline and acid phosphatase) or unstable, LDH is a stable cytoplasmic enzyme present in all cells and rapidly released into the cell culture supernatant upon damage of the plasma membrane. LDH activity can be determined by a coupled enzymatic reaction: LDH oxidizes lactate to pyruvate which then reacts with tetrazolium salt INT to form formazan. The increase in the amount of formazan produced in culture supernatant directly correlates to the increase in the number of lysed cells. The formazan dye is water-soluble and can be detected by spectrophotometer at 500 nm. The LDH-cytotoxicity assay is sensitive, convenient, and precise, and is applicable to a variety of cytotoxicity studies. Assay takes ~0.5-1 hr.

Summary:

- Detection method- Absorbance (500 nm)

- Sample type- Cell culture (adherent and suspension)

- Species reactivity- Mammalian

- Applications- This assay provides a convenient and non-radioactive alternative to the [3H]-thymidine and the [51Cr] release assays The assay is based on measurement of activity of lactate dehydrogenase (LDH) which is a stable enzyme normally found in the cytosol of all cells but rapidly releases into the supernatant upon damage of plasma membrane.

Kit components:

- Catalyst, Lyophilized

- Dye Solution

Features and Benefits:

- Simple one-step procedure; takes less than 1 hour

- Fast, sensitive and convenient

Shipping Conditions: Gel pack

Additional Information

Name LDH-Cytotoxicity Assay Kit
Related Product Names LDH-Cytotoxicity Assay Kit, cytotoxicity assay, LDH assay kit, LDH assay, cytotoxicity
Datasheets / Downloads GWB-AXR194 Datasheet
Format Assay Kit
Storage -20C
Intended Use Research Use Only