Anti Deamidated Gliadin Peptide (DGP) IgG (GWB-521207)


96 Wells


Introduction Celiac disease, also known as gluten sensitive enteropathy is primarily a disease of the infant organism. It is caused by a hypersensitivity reaction in response to gliadin, a protein being present in many cereals. This, non IgE mediated food allergy leads to massive malabsorption disturbances and is characterized by a complete atrophy of the villi and a hyperplasia of the crypts of the upper intestine. Accordingly patients suffering from celiac disease must maintain a gluten free diet for the rest of their life. Gliadins are proteins containing high amounts of the amino acids prolin and glutamine. These proteins belong to the nutritive tissue of the grain seeds of wheat, oat, barley and rye and are responsible for the baking properties of the flour. Due to the possibilities of the highly specific and sensitive serological determination of IgG and IgG antibodies against DGP the invasive procedures of biopsies can be given up. In the past several biopsies have been done with patients when celiac disease was suspected, after a period of a gluten-free diet and also after a specific gluten challenge. DGP antibodies titer has been proved to correlate very well with the morphological appearance of the mucosa of the upper intestine. It has been well documented that DGP antibodies level fall very quickly after a gluten free diet has begun and rise immediately after restoring gluten to the diet. Thus the serological test represents a reliable method to monitor patients, and in particular children and teenagers, for their adherence to the gluten-free diet.
Intended Use Anti-Deamidated Gliadin Peptide (DPG) IgG kit is a solid phase enzyme immunometric assay (ELISA) designed for the quantitative measurement of IgG class antibodies directed against deamidated Gliadin peptides (DGP) in human serum or plasma. Anti-Deamidated Gliadin Peptide (DPG) IgG is intended for laboratory use only.
Principle of the Assay Anti-Deamidated Gliadin Peptide (DPG) IgG test is based on the binding of present antibodies in calibrators, controls or predilutedpatient samples on the syntetic deamidated Gliadin peptides (DGP) coated on the inner surface of the wells. After a 30 minutesincubation the microplate is washed with wash buffer for removing non-reactive serum components.An anti-human-IgG horseradish peroxidase conjugate solution recognizes IgG class antibodies bound to the immobilized antigens.After a 30 minutes incubation any excess enzyme conjugate, which is not specifically bound is washed away with wash buffer.A chromogenic substrate solution containing TMB is dispensed into the wells. After 15 minutes of incubation the color developmentis stopped by adding the stop solution. The solutions color changes into yellow. The amount of color is directly proportional to theconcentration of IgG antibodies present in the original sample.

Additional Information

Name Anti Deamidated Gliadin Peptide (DGP) IgG (GWB-521207)
Related Product Names Anti Deamidated Gliadin Peptide Immunoglobulin G ELISA Kit, Anti DGP Immunoglobulin G ELISA, Anti DGP IgG ELISA, Anti Deamidated Gliadin Peptide IgG ELISA, Celiac disease ELISA, Gastro Enterology Assay
Molecular Weight 0.5
Concentration Lot Specific
Application Enzyme immunoassay for the quantitativedetermination of auto-antibodies against deamidated Gliadin peptides (DGP) in human serum or plasma
Reactivity Human
Storage Store all the kit reagents at 2-8°C. Do not freeze. Reagents are stable until the expiration date when stored and handled as directed Unused antigen coated microwell strips should be resealed securely in the foil pouch containing desiccants and stored at
Datasheets/Manuals Printable manual for GWB-521207
Intended Use Research Use Only